Single passage in mouse organs enhances the survival and spread of Salmonella enterica

Intravenous inoculation of Salmonella enterica serovar Typhimurium into mice is a prime experimental model of invasive salmonellosis. The use of wild-type isogenic tagged strains (WITS) in this system has revealed that bacteria undergo independent bottlenecks in the liver and spleen before establishing a systemic infection. We recently showed that those bacteria that survived the bottleneck exhibited enhanced growth when transferred to naive mice. In this study, we set out to disentangle the components of this in vivo adaptation by inoculating mice with WITS grown either in vitro or in vivo. We developed an original method to estimate the replication and killing rates of bacteria from experimental data, which involved solving the probability-generating function of a non-homogeneous birth–death–immigration process. This revealed a low initial mortality in bacteria obtained from a donor animal. Next, an analysis of WITS distributions in the livers and spleens of recipient animals indicated that in vivo-passaged bacteria started spreading between organs earlier than in vitro-grown bacteria. These results further our understanding of the influence of passage in a host on the fitness and virulence of Salmonella enterica and represent an advance in the power of investigation on the patterns and mechanisms of host–pathogen interactions.     

The structure solution of the F-type i-ZnMgHf from the X-ray diffraction

ABSTRACT

We report a successful growth of the ZnMgHf F-type icosahedral quasicrystal in the form of faceted single grains. By varying the heat treatment parameters we were able to obtain single crystals with a quality suitable for a single crystal X-ray diffraction which was conducted in a synchrotron facility. Ab initio structure solution by a charge flipping algorithm manifests the existence of two types of Bergman clusters. Clusters are differentiated by the preferential occupation of the high-symmetry positions by hafnium in a rhombic triacontahedron, being the outer shell of the atomic cluster.

This paper is part of a Thematic Issue on The Crystallographic Aspects of Metallic Alloys.     

Nightingallery: theatrical framing and orchestration in participatory performance

The Nightingallery project encouraged participants to converse, sing, and perform with a musically responsive animatronic bird, playfully interacting with the character while members of the public could look on and observe. We used Nightingallery to frame an HCI investigation into how people would engage with one another when confronted with unfamiliar technologies in conspicuously public, social spaces. Structuring performances as improvisational street theatre, we styled our method of exhibiting the bird character. We cast ourselves in supporting roles as carnival barkers and minders of the bird, presenting him as if he were a fantastical creature in a fairground sideshow display, allowing him the agency to shape and maintain dialogues with participants, and positioning him as the focal character upon which the encounter was centred. We explored how the anthropomorphic nature of the bird itself, along with the cultural connotations associated with the carnival/sideshow tradition helped signpost and entice participants through the trajectory of their encounters with the exhibit. Situating ourselves as secondary characters within the narrative defining the performance/use context, our methods of mediation, observation, and evaluation were integrated into the performance frame. In this paper, we explore recent HCI theories in mixed reality performance to reflect upon how genre-based cultural connotations can be used to frame trajectories of experience, and how manipulation of roles and agency in participatory performance can facilitate HCI investigation of social encounters with playful technologies.     

Optical investigation of the behavior of the electric arc and the metal transfer during vacuum remelting of a Ti alloy

High-speed camera imaging and optical emission spectroscopy have been used for investigating the structure of the electric arc and the transfer mechanisms of the liquid metal during vacuum arc remelting (VAR) of Ti alloys. The arc exhibited a similar operating regime to that described in the previous literature for the case of Inconel 718 and zirconium alloy electrodes. The arc behaved in a diffuse mode with many separate and rapidly moving cathode spots. Several parameters of the cathode spots, including their current, size and apparent velocity were evaluated. The application of an external axial magnetic field tended to encourage the cathode spots to locate themselves on the base of the electrode. A large density ratio of Ti⁺ ions and Ti atoms in the interelectrode plasma was evaluated, suggesting that the plasma was strongly ionized. The calculated excitation temperature of Ti⁺ ions (1–1.2 eV) was about 1.5–2 times greater than that obtained for Ti atoms. The transfer mechanisms of the drops of liquid metal might be classified into three main modes depending on the gap length: drop falling, drip short and drop erosion induced by the cathode spots. The importance of the influence of the arc on the metal transfer mechanisms was inversely related to the gap length.     

3D line-support grid flattening for more accurate geostatistical reservoir population with petrophysical properties

Reservoir models are essential if we need to clearly understand the fossil resources and, hence, to make better use of them. Feeding these models with physical properties on the basis of wells data is a key step in their construction. Line-support (LS) grid is the most popular grid in reservoir engineering, it is massively used for reservoir simulations. In the current methods used to populate with properties the LS grid of a reservoir unit, a Cartesian grid of equivalent size (in each direction), obtained by averaging the edge lengths, is first of all completed. The properties calculated in this way are then transferred as they are into the initial LS grid, because there is cell-for-cell correspondence. This leads to distortion of the Cartesian grid, making it fit the shape of the LS grid. This has the effect of altering calculations of correlation distances between well markers in geostatistical population simulations. Consequently, this primarily induces distortions on the simulated bodies. To resolve this problem, in this paper, we propose innovative methods for a “smooth” conversion from the LS grid of the structural space to the Cartesian grid of the geostatistical population space. The basic principle is to calculate the correlation distances between wells on the basis of “quasi-isometric” flattening of the stratigraphic unit LS grid in the population space. This same flattening technique is then used for inverse transfer of the properties from the population space to the structural space.     

Assessing the capabilities of the zonal model to predict the isothermal airflow induced by a linear ceiling diffuser

The objective of this work is to evaluate the quality of the zonal airflow predictions compared to those provided by computational fluid dynamics (CFD) tools for an isothermal airflow induced in a room by a linear ceiling diffuser. This comparative analysis was conducted for a typical rectangular office designed for two people, considering two different arrangements of obstacles within the room volume. The ventilation was provided by a four-way ceiling diffuser type with large aspect ratio slots, the outlet integrated into it, operating in a three-way mode with slot Reynolds number of 2700. As a result, the airflow was transitional or weakly turbulent. The airflow patterns obtained with the zonal models were first compared qualitatively with the CFD computations. A quantitative comparison based on the mean velocities at the interface of the zonal grid was then carried out. The characteristic features of the zonal predictions are shown and the limitations of the zonal and CFD approaches are discussed.